How To Draw Blood From Mice

Multiple samples can be taken daily by alternating sides. Withdraw needle after blood has been collected.

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25g mouse x 1 025mL or 250uL maximum blood removal No more than 15 of the animals body weight in two weeks 14 days For example.

How to draw blood from mice. The tail can be gently stroked from the base of the tail toward the tail to encourage blood flow. Blood collection from the submandibular vein is the preferred blood collection technique for mice. 200g rat x 15 30mL maximum over 14 days.

For example a 25 gram mouse has a blood volume of approximately 2 ml so no more than 200 l of blood can be removed at a single bleeding without scientific justification and. If the maximum amount 1 of body weight in blood as defined above must be drawn all at once or via multiple draws over a 24-hour period replacement fluids sterile 09 saline or Lactated Ringers Solution should be administered. Place the mouse in a restraining tube so its head is covered and its hind legs are free.

If no blood appears slowly withdraw the needle so that it remains just under the skin or the diaphragm and redirect in a slightly different direction. Use a dry piece of gauze to apply pressure to the puncture site while releasing pressure on the upper thigh until bleeding stops. Ml of blood from an adult mouse depending on the fre-quency of bleeding.

The blood volume of a mouse is approximately 8 of the body weight. Blood Collection methods in Mice and Rats unanesthetized. For a 25 g mouse this is equivalent to 145-150 l per week.

The techniques described below are recommended by ACS staff for survival. Blood Collection from the Saphenous Vein Warming the mouse immediately prior to blood collection will increase blood flow considerably. Blood flow Blood is drawn from a small vascular bundle at the back of the jaw.

Maximum three samples can be taken per leg and 01 to 03 ml of blood can be collected per sample. This training video from Imperial College shows animal technologists how to take blood from a mouse or rat. Perform secondary method of euthanasia to ensure that animal is deceased.

Collect blood either by touching a capillary tube to the bead of blood or by allowing the blood to drop into a collection tube. If sampling will occur every 2 weeks up to 10 of the total blood volume may be drawn or 8 lg 4. Submandibular or Facial veinartery Sampling.

Tail veins saphenous vein facial veins orbital venous sinus requires anesthesia see alternative listed below terminal cardiac blood collection under anesthesia. Than 2 draws per side 25 Clinical chemistry values may be higher with this method than with the retro-orbital plexus route. For example for a mouse weighing 25 grams the maximum allowable blood collection may not exceed 025 grams or 025 ml.

This method allows for maximum allowable sample volume with minimal trauma to the animal. Blood Collection from the Lateral Saphenous Vein in Mice and Rats SOP Page 5 of 5 10. If blood stops flowing slowly rotate needle or move it slightly in or out.

Simply applying a sterile gauze pad or tissue with a little compression for less than a minute will stop the bleeding. This technique must be. Sample may be a mixture of venous and arterial blood.

The problem with this method is that control of. There are a variety of methods that are utilized to collect blood from mice. A mouse weighing 25 g would therefore have a total blood volume TBV of approximately 585 mlkg x 0025 kg 146 ml.

Repeated sampling is possible by alternating sides of the face. The puncture site is caudal to the small cowlick Not recommended for serial draws. How to decide on the most appropriate blood sampling technique for mice.

Open in a separate window. After the mouse is released it will groom and within a short time will show no evidence of the blood draw. Remove the mouse or rat from the restrainer and place it.

Volumes should be drawn. It includes guidance on handling shaving off the fur locating the vein and taking a blood. On average mice have around 585 ml of blood per kg of bodyweight.

After the sample collection gentle pressure is applied with finger for 2 minutes to stop bleeding Figure 6. The maximum blood volume that may be drawn per week is no more than 75 of the total blood volume or 6 lg. Saphenous Sampling medial or lateral approach.

The two tables below are designed to assist in determining the amount of blood to. After 20 to 30 minutes blood sample is collected slowly by using 22G needle. For a 25 g mouse this is equivalent to 200 l every 2 weeks.

Place a lamp over the cage for five minutes or place the cage on a heating pad on the lowest setting.

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