Once sedated shave the fur from the mid-cervical area on the ventrolateral aspect of the neck. Assuring that blood flow has stopped hemostasis is of upmost importance after collecting a blood sample.
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A video demonstrating how to draw blood from poultryMusic used byAnd Awaken - Stings by Kevin MacLeod is licensed under a Creative Commons Attribution lice.
How to draw blood from rabbit. Large volumes of blood can be collected directly from the heart of anesthetized rabbits as a terminal event. No more than 1 of the animals blood volume in one collection or over a 24 hour period. It is also the vein of choice on small rabbits.
Considerations One of the most common and least invasive methods of blood collection in the rabbit. The total volume of blood that can be safely collected typically ranges. Describes procedure for the collection of blood via the marginal ear vein in rabbits.
The author prefers to use the lateral saphenous vein for routine blood collection in rabbits. 10072011 Restraint involves firmly holding the rabbit on its side and extending the rear leg while simultaneously holding pressure around the stifle to facilitate blood collection. The technique is similar to that described for mice and rats however a larger needle and syringe 15in 18 gauge.
4 ml from a 400 gram rat 40 ml from a 4 kg rabbit. The jugular vein is a nice vein to use as long as your patient can be sedated. The tail can be gently stroked from the base of the tail toward the tail to encourage blood flow.
Total blood volume of a rabbit is 60 mlkg or 60 of total body weight BW. The auricular artery can also be accessed for larger amounts of blood but there is a greater risk of hematoma or bruising. Death must be confirmed at the completion of the procedure by administering pentobarbital 120 mgkg IV.
The patient is maintained in a normal standing position with the rear leg held extended above the hock and the vein raised at knee level Figure 1. Maximum three samples can be taken per leg and 01 to 03 ml of blood can be collected per sample. Safety How YouTube works Test new features Press Copyright Contact us Creators.
Open in a separate window. Safety How YouTube works Test new features Press Copyright Contact us Creators. Any of the large veins around the edge of the ear or the central artery can yield a good supply of blood.
For multiple blood draws separated by a period of weeks a maximum of 1 of the animals body weight can be removed ie 02 ml from a 20 gram mouse. If necessary the rabbit should be wrapped in a towel bunny burrito style. After 20 to 30 minutes blood sample is collected slowly by using 22G needle.
21082011 The rabbit has a relatively short prothrombin time and whole blood quickly clots at room temperature. If necessary the rabbit can be wrapped gently in a towel. The estimated volume at exsanguination is approximately half of the total blood volume.
Most rabbits will sit sternal and let you extend out one of their hind legs for the blood draw. 15042013 About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy. After the sample collection gentle pressure is applied with finger for 2 minutes to stop bleeding Figure 6.
No more than 75 45 ml kg BW of total blood volume can be collected in a single or multiple draws over a week period. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy. Clot blood samples must be made prior to collection so that proper blood draw tubes will be used.
To achieve hemostasis place gentle pressure over the site of blood collection to stop the bleeding. Let the blood sit for 30 minutes to one hour at room temperature to clot before spinning and separating. To make the rabbit feel more comfortable the assistant can rub the rabbits forehead with one hand while holding off the vein at the base of the ear with the other.
If needed the rabbit may also be restrained in a towel with the head covered. To minimize the risk of clot formation it can be helpful to pre-heparinize the needle and syringe by drawing heparin into the needle and expelling the excess from the hub. 20 cc should be used.
This video is about getting a blood sample on the marginal ear vein on a rabbit. Serum needs clot time A serum separator tube SST tiger top tube. It is a good idea to have a towel lightly draped over the patient.
Collect blood either by touching a capillary tube to the bead of blood or by allowing the blood to drop into a collection tube. Marginal ear vein. 25072011 The total blood volume can be calculated as approximately 6 of normal body weight.
Restraint and anesthesia for blood draws.
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