How To Collect Blood From Rabbit

27022020 Blood is collected from most of the small rodents via lateral saphenous vein lateral tail veins ventral tail artery rats and jugular veins. You can also get a more than ample sample from the ear on your larger breeds which is.

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Frequency of blood collection is important.

How to collect blood from rabbit. Depending on the size of the rabbit and the frequency of sampling 05 - 10 ml of blood can be collected. Using clippers remove the hair from the ear. Use a suitable method to dilate the vein or artery to aid visualisation and needleentry.

Avoid the use of xylene for this purpose. The urethra of the female rabbit opens along the wall of the vagina. Once blood starts to flow place receptacle under the needle for blood collection.

Describes procedure for the collection of blood via the marginal ear vein in rabbits. Up to eight samples can be collected in any 24-hour period depending on sample volume and scientific justification. Restraint methods and anesthesia used to collect blood on research animals must be described and approved in the animal protocol.

Examples of restraint devices include rodent restraint tubes surgical towel or decapicones. Terminal blood collection by cardiac puncture can only be performed under anesthesia. This can cause certain materials in the vagina to mix with the urine that is being released.

If necessary the rabbit should be wrapped in a towel bunny burrito style. Once in two weeks is ideal for nonrodents. Central Ear Artery up to 20 mL The rabbit is restrained state method of restraint and the ear is shaved with an electric clipper and cleaned with three alternating scrubs of 70 alcohol and betadine.

Rodent Mouse and Rat Procedures b. Adequate training is required for blood collection using any method in any species. Rabbits are unique in having very accessible options on their ears for blood collection.

Generally one person can perform mouse procedures. 14082019 Sometimes the blood you see in your rabbits urine may not be originating from the urinary system. The vein is normally occluded distally away from the animal before the needle is inserted.

The central auricular artery is one of the most commonly used sites for blood collection and most rabbits. Penetrate the vessel with a hypodermic needle and either collect the blood from thepoint of puncture or leave the needle in place if this technique is used it is best touse a butterfly. One to restrain the animal and one to collect blood.

A 4 kg rabbit is calculated to have a total blood volume of 224 ml 56 mlkg x 40 kg. In general blood sample is withdrawn from venous arterial blood vessels or heart chambers. Any of the large veins around the edge of the ear or the central artery can yield a good supply of blood.

The auricular artery can also be accessed for larger amounts of blood but there is a greater risk of hematoma or bruising. 21082011 To minimize the risk of clot formation it can be helpful to pre-heparinize the needle and syringe by drawing heparin into the needle and expelling the excess from the hub. If the vessel collapses gently stroke the ear until the vessel.

Rabbit and Guinea Pig Procedures 4. This video is about getting a blood sample on the marginal ear vein on a rabbit. To make the rabbit feel more comfortable the assistant can rub the rabbits forehead with one hand while holding off the vein at the base of the ear with the other.

Considerations One of the most common and least invasive methods of blood collection in the rabbit. Purpose This document outlines the preferred methods for collecting blood from animals and blood collection volume and frequency limits. 08012020 Restraint and anesthesia for blood draws.

Blood Collection Limits 3. A 1 to 1-12-inch needle ranging in size from 22gauge to 20- gauge is recommended for blood collection. This position can hamper breathing so care must bepaid at all times.

Non-terminal blood collection without additional monitoring see below should be limited to 10 of the total circulating blood volume on a single collection or every 2 week period for serial collections. 10072011 Restraint involves firmly holding the rabbit on its side and extending the rear leg while simultaneously holding pressure around the stifle to facilitate blood collection. Central Artery Place the rabbit in a rabbit restrainer.

You can use either the central artery or the marginal vein for a blood draw. Some techniques for rats may require two people. Example Using mean blood volume table below.

25072011 025 to 05 mgkg SQ or IM. For blood collection from the jugular vein the rabbit is maintained with its front legs overthe edge of a table and the head extended. The best results are obtained when administered 1015 minutes prior to blood - collection.

The total volume of blood that can be safely collected typically ranges from 05 to 10 body weight. Other procedures will require a restraint device or light anesthesia.

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