How To Draw Blood On A Rabbit

21082011 The rabbit has a relatively short prothrombin time and whole blood quickly clots at room temperature. A 23G needle is inserted into the blood vessel and blood is collected using a capillary tube or a syringe with a needle.

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Marginal ear vein.

How to draw blood on a rabbit. Rabbit Estimated Circulating Blood Volume 56mlkg 44-70mlkg a. Size of the rabbit YES The rabbit is anesthetized and blood samples are taken from the heart using a 19-21 gauge needle preferably the ventricle which can be accessed either via the left side of the chest through the diaphragm from the top of the sternum or via a thoracotomyBlood will be withdrawn slowly to prevent the heart from collapsing. Serum needs clot time A serum separator tube SST tiger top tube.

Let the blood sit for 30 minutes to one hour at room temperature to clot before spinning and separating. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy. Personal Protective Equipment PPE and Hygiene a.

Most rabbits will sit sternal and let you extend out one of their hind legs for the blood draw. To achieve hemostasis place gentle pressure over the site of blood collection to stop the bleeding. Safety How YouTube works Test new features Press Copyright Contact us Creators.

To make the rabbit feel more comfortable the assistant can rub the rabbits forehead with one hand while holding off the vein at the base of the ear with the other. Assuring that blood flow has stopped hemostasis is of upmost importance after collecting a blood sample. The jugular vein is a nice vein to use as long as your patient can be sedated.

Once sedated shave the fur from the mid-cervical area on the ventrolateral aspect of the neck. For multiple blood draws separated by a period of weeks a maximum of 1 of the animals body weight can be removed ie 02 ml from a 20 gram mouse. Ensure appropriate PPE is used to protect technician from accidental exposure to blood and other body.

Practical blood sample volumes for laboratory animals domestic species and non-human primates are given in the table below adapted Wolfensohn. If necessary the rabbit can be wrapped gently in a towel. If needed the rabbit may also be restrained in a towel with the head covered.

To minimize the risk of clot formation it can be helpful to pre-heparinize the needle and syringe by drawing heparin into the needle and expelling the excess from the hub. The tail can be gently stroked from the base of the tail toward the tail to encourage blood flow. The author prefers to use the lateral saphenous vein for routine blood collection in rabbits.

Lloyd 2003 Handbook of Laboratory Animal Management and Welfare 3rd Edition. 15042013 About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy. 25072011 The total blood volume can be calculated as approximately 6 of normal body weight.

Safety How YouTube works Test new features Press Copyright Contact us Creators. It is a good idea to have a towel lightly draped over the patient. Restraint and anesthesia for blood draws.

CBV 3kg x 56mlkg 168ml. Safety How YouTube works Test new features Press Copyright Contact us Creators. If necessary the rabbit should be wrapped in a towel bunny burrito style.

In case of difficulties 05 to 1 cm of surface of the skin is cut open and the vein is pricked with bleeding lancet or needle and blood is collected with a capillary tube or a syringe with a needle. Maximum daily withdrawal without fluid supplementation 001 x 168ml 17ml II. Species Reference weight g Blood volume mlkg Total blood volume TBV normal adult ml Safe volume for single bleed ml Bleed out.

Clot blood samples must be made prior to collection so that proper blood draw tubes will be used. 10072011 Restraint involves firmly holding the rabbit on its side and extending the rear leg while simultaneously holding pressure around the stifle to facilitate blood collection. The estimated volume at exsanguination is approximately half of the total blood volume.

The patient is maintained in a normal standing position with the rear leg held extended above the hock and the vein raised at knee level Figure 1. 4 ml from a 400 gram rat 40 ml from a 4 kg rabbit. Any of the large veins around the edge of the ear or the central artery can yield a good supply of blood.

Collect blood either by touching a capillary tube to the bead of blood or by allowing the blood to drop into a collection tube. It is also the vein of choice on small rabbits. 14112017 About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy.

The total volume of blood that can be safely collected typically ranges.

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